橙橙小狐狸
For the yellow or orange FITC tasted crystalline powder, soluble in water and alcohol solvent. Have two kinds of optical isomers, Ⅰ type in efficiency, stability and protein are more excellent adhesion, etc. For 389.4 FITC molecular weight, the maximum absorption wavelength for 490-495nm, maximum emission wavelength for 520-530nm, presents the bright green fluorescence. In the cold dark FITC dry can save years, is the most widely used fluorescent element. Its main advantage is sensitive to yellow eye, usually less than the green fluorescence sliced red specimens. With FITC markers of antibody], peptides, polysaccharide substances such as] has been widely used in various biological research.FITC have two points, including A in optical isomers efficiency, stability, and protein better ability, etc, in cold dark, dry place for many years, can save another sensitive to yellow-green due to human, usually less than the green fluorescence sliced specimen FITC become red, so the most widely used fluorescent element. With FITC markers of antibody, peptides, polysaccharide substances are widely used in all kinds of biological research.In recent years, the research on drug carrier, also often use fluorescent material. Fluorescent substance will introduce drug carrier methods are: (1) through chemical combination method of fluorescent substance made with polymer lateral-torsion coupling, after the lateral-torsion coupling into drug preparation of polymer particle or directly connected to fluorescent material surface preparation of good decoupling particles. (2) direct will mark or fluorescent substance into the bag contained nanoparticles drug.
小蝴蝶飞不过
Isothiocyanates were fluorescein isothiocyanate fluorescent element (FITC) is tasted, yellow or orange to crystalline powder, soluble in water and alcohol solvent. Have two kinds of optical isomers, Ⅰ type in efficiency, stability and protein are more excellent adhesion, etc. For 389.4 FITC molecular weight, the maximum absorption wavelength for 490-495nm, maximum emission wavelength for 520-530nm, presents the bright green fluorescence. In the cold dark FITC dry can save years, is the most widely used fluorescent element. Its main advantage is sensitive to yellow eye, usually less than the green fluorescence sliced red specimens. With FITC markers of antibody], peptides, polysaccharide substances such as] has been widely used in various biological research.FITC have two points, including A in optical isomers efficiency, stability, and protein better ability, etc, in cold dark, dry place for many years, can save another sensitive to yellow-green due to human, usually less than the green fluorescence sliced specimen FITC become red, so the most widely used fluorescent element. With FITC markers of antibody, peptides, polysaccharide substances are widely used in all kinds of biological research.In recent years, the research on drug carrier, also often use fluorescent material. Fluorescent substance will introduce drug carrier methods are: (1) through chemical combination method of fluorescent substance made with polymer lateral-torsion coupling, after the lateral-torsion coupling into drug preparation of polymer particle or directly connected to fluorescent material surface preparation of good decoupling particles. (2) direct will mark or fluorescent substance into the bag contained nanoparticles drug. 异硫氰酸isothiocyanate角膜荧光素(牛)品、黄色和橙色到结晶粉末,易溶于水、醇溶剂。有两种类型的光学异构体,Ⅰ效率、稳定性和蛋白质更优异的附着力,等等。牛血清白蛋白分子量,为389.4的最大吸收波长为490 - 495nm、最大发射波长为520 - 530nm,呈现出明亮的绿色荧光。在寒冷的黑暗的牛,干可节省是目前应用最广泛的荧光素。它的主要优点是敏感的,黄色的眼,通常小于绿色荧光红标本。抗体标志物与牛,肽、多糖物质如]已广泛应用于各种生物学的研究。 牛有两个分,其中一个在光学异构体效率、稳定性、蛋白质和较强等,黑暗和寒冷、乾燥的地方很多年了,能拯救他人敏感,黄绿色,由于人类,通常小于绿色荧光切片样品变红牛,所以使用最广泛的荧光素。牛血清标记抗体,用多肽、多糖物质被广泛应用于各种各样的生物研究。 近年来的研究、药物载体,也经常用荧光材料。荧光物质将介绍药物载体的方法是:(1),通过化学方法组合的荧光物质与聚合物耦联后,药物制剂耦联到聚合物粒子或直接连接到荧光材料的表面准备好的解耦的粒子。(2)直接将标志或荧光物质进袋里装的纳米药物。不知道能不能过关
金色年华119
免疫荧光测定抗原抗体反应后，利用特殊仪器测定荧光强度而推算被测物浓度的检测方法⑴荧光物质1）荧光色素许多物质都可产生荧光现象，但并非都可用作荧光色素。只有那些能产生明显的荧光并能作为染料使用的有机化合物才能称为免疫荧光色素或荧光染料。常用的荧光色素有：⑴异硫氰酸荧光素（fluoresceinisothiocyanate，FITC）为黄色或橙黄色结晶粉末，易溶于水或酒精等溶剂。分子量为389.4，最大吸收光波长为490--495nm，最大发射光波长520--530nm，呈现明亮的黄绿色荧光，结构式如下：有两种同分异结构，其中异构体Ⅰ型在效率、稳定性、与蛋白质结合能力等方面都更好，在冷暗干燥处可保存多年，是应用最广泛的荧光素。其主要优点是：①人眼对黄绿色较为敏感，②通常切片标本中的绿色荧光少于红色。⑵四乙基罗丹明（rhodamine，RIB200）为橘红色粉末，不溶于水，易溶于酒精和丙酮。性质稳定，可长期保存。结构式如下：最大吸收光波长为570nm，最大发射光波长为595～600nm，呈橘红色荧光。⑶四甲基异硫氰酸罗丹明（tetramethylrhodamineisothiocyanate，TRITC）结构式如下：最大吸引光波长为550nm，最大发射光波长为620nm，呈橙红色荧光。与FITC的翠绿色荧光对比鲜明，可配合用于双重标记或对比染色。其异硫氰基可与蛋白质结合，但荧光效率较低。⑵其他荧光物质
rachelliu1
DAPI细胞染色液(DAPI Staining Solution)是适用于常见细胞和组织细胞核染色的染色液。DAPI，即2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride，也称DAPI dihydrochloride，分子式为C16H15N5 · 2HCl ，分子量为350.25。可以穿透细胞膜的蓝色荧光染料，和双链DNA结合后可以产生比DAPI自身强20多倍的荧光，灵敏度高于EB。 DAPI染色常用于细胞凋亡检测，染色后用荧光显微镜观察或流式细胞仪检测。DAPI也常用于普通的细胞核染色以及某些特定情况下的双链DNA染色。DAPI的最大激发波长为340nm，最大发射波长为488nm，DAPI和双链DNA结合后，最大激发波长为364nm，最大发射波长为454nm。本DAPI染色液可以直接用于固定细胞或组织的细胞核染色，亦可以按照具体实验要求，稀释到相应浓度后进行染色。一般推荐工作浓度为0.5ug～10ug/ml。详细请查看百博生物：
猪猪爱吃草
FITC (fluorescein isothiocyanate, FITC) pure yellow or orange yellow crystalline powder, soluble in water and alcohol solvent. There are two isomers, one isomer type Ⅰ efficiency, stability and protein binding, etc. are better. FITC molecular weight of 389.4, the maximum absorption wavelength for the 490 ~ 495nm, the maximum emission wavelength for the 520 ~ 530nm, a bright yellow-green fluorescence. FITC in the dark cold can be stored dry for many years, is currently the most widely used fluorescein. Its main advantage is the human eye is more sensitive to yellow-green, usually biopsy samples less than the green fluorescent red.FITC labeled with various antibodies], peptide, polysaccharide] and other substances have been used in a wide variety of biological research.FITC, there are two isomers, one isomer A in terms of efficiency, stability, and protein binding capacity, etc. are better in the cold dark, dry place can be stored for many years, the other as a result of the human eye is more sensitive to yellow-green, biopsy specimens are usually in the red than green fluorescence, FITC therefore become the most widely used fluorescein. FITC labeled with a variety of antibodies, peptides, polysaccharides and other substances has been widely used in a variety of biological research.In recent years the study of drug carrier, and is often the use of fluorescent substances. The introduction of the fluorescent material of drug delivery methods are: (1) by chemical method combined with fluorescent material so that coupled with the polymer and then coupling of the polymer after the drug-loaded particles prepared or coupled directly to the fluorescent material in the preparation of good the surface of the particles. (2) directly to the fluorescent marker substance or package containing the drugs into nanoparticles.
